Journal
OPTICS EXPRESS
Volume 26, Issue 22, Pages 28312-28322Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.26.028312
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- Deutsche Forschungsgemeinschaft
- Open Access Publication Fund of Bielefeld University
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Picosecond optical parametric oscillators (OPOs) with broad wavelength tunability are frequently used as light sources in hyperspectral coherent Raman scattering (CRS) microscopy. We investigate how changes in the pulse length during OPO wavelength tuning of the pump beam affect hyperspectral CRS imaging. We find that significant distortions of the resulting CRS spectra occur if the OPO is operated without monitoring pulse length variations. By utilizing a custom-written MATLAB based control program to counteract changes in pulse length, normalized and reproducible data sets can be acquired. We demonstrate this by comparing hyperspectral data obtained from pure substances, as well as relevant biological specimens. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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