Journal
OPTICS EXPRESS
Volume 27, Issue 2, Pages 1497-1506Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.27.001497
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Funding
- Ministry of Science Technology of Taiwan (MOST) [105-2119-M-001-026-MY2, 103-2113-M-001-033-MY2]
- Academia Sinica [CDA-107-M08]
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We present a novel method used to implement tiling lattice light sheets (LLS) in lattice light sheet microscopy (LLSM) on regular LLS microscopes without changing the LLS microscope hardware. A LLS is tiled by applying binary phase maps acquired from off-center cross-sections of the corresponding optical lattice to the binary SLM used in LLS microscopes, by which a thin LLS can be tiled to image large specimens while maintaining the 3D imaging ability in the entire field of view. We investigate the method via numerical simulations and experiments, and demonstrate the method by imaging fluorescent particles embedded in agarose gel and expanded cells in the dithered mode of LLSM. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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