Journal
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
Volume 1849, Issue 11, Pages 1354-1362Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbagrm.2015.10.003
Keywords
RNA polymerase; Nuclear import; RNA pol II biogenesis; Iwr1; Rtr1; Yeast
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Funding
- Spanish Ministry of Education and Science [BFU2011-23501/BMC]
- F.P.I. programme Cinc Segles at the Universitat de Valencia
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Recent studies suggest that RNA polymerase II (Pol II) has to be fully assembled before being imported into the nucleus, while other reports indicate a distinct mechanism to import large and small subunits. In yeast, Iwr1 binds to the holoenzyme assembled in the cytoplasm and directs its nuclear entry. However, as IWR1 is not an essential gene, Iwr1-independent pathway(s) for the nuclear import of Pol II must exist. In this paper, we investigate the transport into the nucleus of several large and small Pol II subunits in the mutants of genes involved in Pol II biogenesis. We also analyse subcellular localization in the presence of drugs that can potentially affect Pol II nuclear import. Our results show differences in the cellular distribution between large and small subunits when Pol II biogenesis was impaired. Our data suggest that, in addition to the fully assembled holoenzyme, Pol II subunits can be imported to the nucleus, either independently or as partial assemblies, through different pathways, including passive diffusion for the small subunits. (C) 2015 Elsevier B.V. All rights reserved.
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