Journal
ACS NANO
Volume 10, Issue 5, Pages 4989-4996Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.6b01339
Keywords
DNA origami; DNA binding; conformation change
Categories
Funding
- U.S. National Science Foundation [1055866, 1437301, 1512537]
- Office of Naval Research [N00014-15-1-2707]
- Swedish Research Council VR
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1512537] Funding Source: National Science Foundation
- Div Of Civil, Mechanical, & Manufact Inn
- Directorate For Engineering [1055866] Funding Source: National Science Foundation
- Div Of Civil, Mechanical, & Manufact Inn
- Directorate For Engineering [1437301] Funding Source: National Science Foundation
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DNA origami has received enormous attention for its ability to program complex nanostructures with a few nanometer precision. Dynamic origami structures that change conformation in response to environmental cues or external signals hold great promises in sensing and actuation at the nanoscale. The reconfiguration mechanism of existing dynamic origami structures is mostly limited to single-stranded hinges and relies almost exclusively on DNA hybridization or strand displacement. Here, we show an alternative approach by demonstrating on-demand conformation changes with DNA-binding molecules, which intercalate between base pairs and unwind DNA double helices. The unwinding effect modulates the helicity mismatch in DNA origami, which significantly influences the internal stress and the global conformation of the origami structure. We demonstrate the switching of a polymerized origami nanoribbon between different twisting states and a well-constrained torsional deformation in a monomeric origami shaft. The structural transformation is shown to be reversible, and binding isotherms confirm the reconfiguration mechanism. This approach provides a rapid and reversible means to change DNA origami conformation, which can be used for dynamic and progressive control at the nanoscale.
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