Journal
ACS NANO
Volume 10, Issue 2, Pages 2834-2842Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b07966
Keywords
RNA; surface-enhanced Raman spectroscopy; nanoparticles; plasmonic; label-free
Categories
Funding
- European Research Council [PrioSERS FP7/2014 623527]
- Ministerio de Economia y Competitividad [CTQ2014-59808R]
- Generalitat de Catalunya [2014-SGR-480]
- Medcom Advance SA
- ICREA Funding Source: Custom
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As more biological activities of ribonucleic adds continue to emerge, the development of efficient analytical tools for RNA identification and characterization is necessary to acquire an in-depth understanding of their functions and chemical properties. Herein, we demonstrate the capacity of label-free direct surface-enhanced Raman scattering(SERS) analysis to access highly specific structural information on RNAs at the ultrasensitive level. This includes the recognition of distinctive vibrational features of RNAs organized into a variety of conformations (micro-,fully complementary duplex-,small interfering-and short hairpin-RNAs) or characterized by subtle chemical differences (single-base variances, nucleobase modifications and backbone composition). This method represents a key advance in the ribonucleic acid analysis and will have a direct impact in a wide range of different fields, including medical diagnosis, drug design, and biotechnology, by enabling the rapid, high-throughput, simple, and low-cost identification and classification of structurally similar RNAs.
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