Journal
ACS NANO
Volume 10, Issue 9, Pages 8207-8214Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.6b03759
Keywords
DNA origami; lipid membrane; synthetic porin; ionic current recordings; molecular dynamics
Categories
Funding
- Winton Programme for the Physics of Sustainability
- Gates Cambridge
- Oppenheimer Ph.D. studentship
- ERC Consolidator Grant [Designerpores 647144]
- Early Postdoc Mobility fellowship of the Swiss National Science Foundation
- National Science Foundation [DMR-1507985, PHY-1430124, EEC-1227034]
- XSEDE Allocation grant [MCA05S028]
- Blue Waters petascale supercomputer system (UIUC)
- Marie Sklodowska Curie Actions within the Initial Training Networks Trans location Network [607694]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [1507985] Funding Source: National Science Foundation
- Directorate For Engineering
- Div Of Engineering Education and Centers [1227034] Funding Source: National Science Foundation
- Engineering and Physical Sciences Research Council [1643849] Funding Source: researchfish
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DNA nanotechnology allows for the creation of three-dimensional structures at nanometer scale. Here, we use DNA to build the largest synthetic pore in a lipid membrane: to date, approaching the dimensions of the nuclear pore complex and increasing the pore-area and the conductance 10-fold compared to previous man-made channels. In our design, 19 cholesterol tags anchor a megadalton funnel-shaped DNA origami porin in a lipid bilayer membrane. Confocal imaging and ionic current recordings reveal spontaneous insertion of the. DNA porin into the lipid membrane, creating a transmethbrane pore of tens of nanosiemens conductance. All-atom molecular dynamics simulations characterize the conductance mechanism at the atomic level and independently confirm the DNA porins' large ionic conductance.
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