Journal
ACS NANO
Volume 10, Issue 7, Pages 6769-6781Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.6b02386
Keywords
small molecule; semiconductor polymer dots; dynamic monitoring; fluorescent nanoparticle; in vivo imaging
Categories
Funding
- Key Program of National Natural Science Foundation of China [NSFC 61335001]
- Thousand Young Talents Program
- University of Macau [MYRG2014-00093-FHS, MYRG 2015-00036-FHS]
- Macao government [026/2014/A1, 025/2015/A1, 052/2015/A2]
Ask authors/readers for more resources
Small molecules participate extensively in various life processes. However, specific and sensitive detection of small molecules in a living system is highly challenging. Here, we describe in vivo real-time dynamic monitoring of small molecules by a luminescent polymer dot oxygen transducer. The optical transducer combined with an oxygen-consuming enzyme can sensitively detect small-molecule substrates as the enzyme-catalyzed reaction depletes its internal oxygen reservoir in the presence of small molecules. We exemplify this detection strategy by using glucose-oxidase-functionalized polymer dots, yielding high selectivity, large dynamic range, and reversible glucose detection in cell and tissue environments. The transducer-enzyme assembly after subcutaneous implantation provides a strong luminescence signal that is transdermally detectable and continuously responsive to blood glucose fluctuations for up to 30 days. In view of a large library of oxygen-consuming enzymes, this strategy is promising for in vivo detection and quantitative determination of a variety of small molecules.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available