Journal
NATURE NANOTECHNOLOGY
Volume 14, Issue 2, Pages 184-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41565-018-0336-3
Keywords
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Funding
- Swedish Research Council [2013-5883]
- Swedish Foundation for Strategic Research [FFL12-0219]
- European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme [724872]
- Knut and Alice Wallenberg foundation [KAW2014.0241]
- SFO initiative StatRegen at Karolinska Institutet
- Research Council of Norway through its Centres of Excellence funding scheme [179573]
- Research Council of Norway [230526/F20, 274993/O70]
- Swedish Foundation for Strategic Research (SSF) [FFL12-0219] Funding Source: Swedish Foundation for Strategic Research (SSF)
- European Research Council (ERC) [724872] Funding Source: European Research Council (ERC)
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Although repetitive patterns of antigens are crucial for certain immune responses, an understanding of how antibodies bind and dynamically interact with various spatial arrangements of molecules is lacking. Hence, we introduced a new method in which molecularly precise nanoscale patterns of antigens are displayed using DNA origami and immobilized in a surface plasmon resonance set-up. Using antibodies with identical antigen-binding domains, we found that all the subclasses and isotypes studied bind bivalently to two antigens separated at distances that range from 3 to 17 nm. The binding affinities of these antibodies change with the antigen distances, with a distinct preference for antigens separated by approximately 16 nm, and considerable differences in spatial tolerance exist between IgM and IgG and between low-and high-affinity antibodies.
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