Journal
ACS NANO
Volume 10, Issue 9, Pages 8394-8402Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.6b03159
Keywords
mechanism; dsDNA; ssDNA; translocation; transport; barrier
Categories
Funding
- European Research Council [260884] Funding Source: Medline
- European Research Council (ERC) [260884] Funding Source: European Research Council (ERC)
Ask authors/readers for more resources
Many important processes in biology involve the translocation of a biopolymer through a nanometer-scale pore. Moreover, the electrophoretic transport of DNA across nanopores is under intense investigation for single-molecule DNA sequencing and analysis. Here, we show that the precise patterning of the ClyA biological nanopore with positive charges is crucial to observe the electrophoretic translocation of DNA at physiological ionic strength. Surprisingly, the strongly electronegative 3.3 nm internal constriction of the nanopore did not require modifications. Further, DNA translocation could only be observed from the wide entry of the nanopore. Our results suggest that the engineered positive charges are important to align the DNA in order to overcome the entropic and electrostatic barriers for DNA translocation through the narrow constriction. Finally, the dependencies of nucleic acid translocation on the Debye length of the solution are consistent with a physical model where the capture of double-stranded DNA is diffusion-limited while the capture of single-stranded DNA is reaction-limited.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available