Journal
NATURE METHODS
Volume 15, Issue 11, Pages 924-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-018-0178-9
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Funding
- Helmholtz association
- German Research Council (DFG)
- Federal Ministry of Education and Research (BMBF)
- German Network for Bioinformatics Infrastructure (HD.HuB as part of de.NBI) [031A537C]
- Helmholtz International Graduate School for Cancer Research (DKFZ, Heidelberg)
- German Center for Infection Research (DZIF) [TTU-HIV 04.803]
- Cystic Fibrosis Foundation Therapeutics (CFFT) [GRIMM15XX0]
- Transregional Collaborative Research Center [TRR179]
- Cluster of Excellence CellNetworks (DFG) [EXC81]
- European Research Council [716058]
- Swiss National Science Foundation
- Biltema Foundation
- CSCS-Swiss National Supercomputing Centre
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Anti-CRISPR proteins are powerful tools for CRISPR-Cas9 regulation; the ability to precisely modulate their activity could facilitate spatiotemporally confined genome perturbations and uncover fundamental aspects of CRISPR biology. We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent Streptococcus pyogenes Cas9 inhibitor, and the LOV2 photosensor from Avena sativa. Coexpression of these proteins with CRISPR-Cas9 effectors enabled light-mediated genome and epigenome editing, and revealed rapid Cas9 genome targeting in human cells.
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