Journal
NATURE METHODS
Volume 15, Issue 12, Pages 1045-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-018-0212-y
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Funding
- Amon Carter Foundation
- Laurie Krauss Lacob Faculty Scholar Fund
- California Institute for Regenerative Medicine [DISC2-08874, LA1_C12-06917]
- Japan Society (JSPS Postdoctoral Fellowship for Overseas Researchers)
- Lucile Packard Foundation for Children's Health
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Scarless genome editing in human pluripotent stem cells (hPSCs) represents a goal for both precise research applications and clinical translation of hPSC-derived therapies. Here we established a versatile and efficient method that combines CRISPR-Cas9-mediated homologous recombination with positive-negative selection of edited clones to generate scarless genetic changes in hPSCs.
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