Journal
NATURE METHODS
Volume 15, Issue 11, Pages 909-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-018-0179-8
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Funding
- KG Jebsen Foundation
- Helse-Sor-Ost
- Novo-Nordisk Foundation
- Norwegian Research Council
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Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we used preparative gel electrophoresis to develop a capture format for WB. Fractions with soluble, size-separated proteins facilitated parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). This pipeline provided the means for large-scale implementation of antibody validation concepts proposed by an international working group on antibody validation (IWGAV).
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