Journal
NATURE METHODS
Volume 15, Issue 11, Pages 932-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41592-018-0175-z
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Funding
- Knut and Alice Wallenberg Foundation [2015.0041]
- Swedish Foundation for Strategic Research [RIF 14-0057, SB 16-0065]
- Swedish Research Council [542-2013-8373]
- Chan Zuckerberg Initiative [2017-174399]
- NIH [1U01MH114812-01]
- Swedish Infrastructure for Computing [SNIC 2016/1-283]
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Global efforts to create a molecular census of the brain using single-cell transcriptomics are producing a large catalog of molecularly defined cell types. However, spatial information is lacking and new methods are needed to map a large number of cell type-specific markers simultaneously on large tissue areas. Here, we describe a cyclic single-molecule fluorescence in situ hybridization methodology and define the cellular organization of the somatosensory cortex.
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