Journal
NATURE CELL BIOLOGY
Volume 21, Issue 1, Pages 72-84Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41556-018-0251-8
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Funding
- Wellcome Trust Strategic Award [107457]
- European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant [766181]
- Deutsche Forschungsgemeinschaft (DFG) [166, FOR 1905]
- Medical Research Council [MC_UU_12010, G0902418, MC_UU_12025, MR/K01577X/1]
- Wellcome Trust [107457, 104924/14/Z/14]
- Oxford internal funds (EPA Cephalosporin Fund)
- Oxford internal funds (John Fell Fund)
- BBSRC [BB/P026354/1] Funding Source: UKRI
- MRC [MC_UU_00008/9] Funding Source: UKRI
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Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.
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