Journal
NATURE BIOTECHNOLOGY
Volume 36, Issue 10, Pages 950-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/nbt.4261
Keywords
-
Categories
Funding
- National Key Research and Development Program of China [2016YFD0101804]
- National Natural Science Foundation of China [31788103, 31420103912]
- National Transgenic Science and Technology Program [2016ZX08010002, 2018ZX0800102B-001, 2018ZX0801002B-002]
- Chinese Academy of Sciences [QYZDY-SSW-SMC030, GJHZ1602]
Ask authors/readers for more resources
Base editors (BEs) have been used to create C-to-T substitutions in various organisms. However, editing with rat APOBEC1-based BE3 is limited to a 5-nt sequence editing window and is inefficient in GC contexts. Here, we show that a base editor fusion protein composed of Cas9 nickase and human APOBEC3A (A3A-PBE) converts cytidine to thymidine efficiently in wheat, rice and potato with a 17-nucleotide editing window at all examined sites, independent of sequence context.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
