Journal
NATURAL PRODUCT RESEARCH
Volume 34, Issue 6, Pages 893-897Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2018.1508144
Keywords
Breast cancer; ginkgo biloba sarcotestas; 2-Hydroxy-6-tridecylbenzoic acid; antiproliferation; AhR
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Funding
- 10-100-1000 Program for High-End Talents Introductionof Liaoning Province in China [521082403-880303-88030312004]
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This study aims to isolate the potential antiproliferative and cytotoxic compounds from ginkgo biloba sarcotestas (GBS) and investigates the underlying mechanism in human MDA-MB-231 and mouse 4T-1 triple-negative breast cancer cells. Our results showed that 2-Hydroxy-6-tridecylbenzoic acid was isolated by cytotoxicity-guided fractionation where different fractions were assessed using MTT assay against MDA-MB-231 and 4T-1 cells. Colony formation assay showed that 2-Hydroxy-6-tridecylbenzoic acid significantly inhibited cell proliferation. The inhibition was associated with the enhancement of cytochrome P450 (CYP) 1B1 expression in a dose- and time-dependent manner and no significant change of CYP1A1 expression by qPCR and Western blot assays in MDA-MB-231 and 4T-1 cells. The mechanism was further demonstrated by the activation of aryl hydrocarbon receptor (AhR) pathway with the upregulation of AhR, AhR nuclear translocator (ARNT) and AhR-dependent xenobiotic response elements (XRE) activity. These findings may have implications for development of anticancer agents containing 2-Hydroxy-6-tridecylbenzoic acid as functional additives.
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