Journal
NANOMEDICINE
Volume 13, Issue 20, Pages 2657-2667Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/nnm-2018-0217
Keywords
albumin; apolipoprotein B-100; dendritic polyglycerol; pNIPAM; pNIPMAM; protein corona; proteomics; thermoresponsive nanogels
Funding
- Bundesministerium fur Bildung und Forschung (BMBF) through the NanoMatFutur award [13N12561]
- Helmholtz Virtual Institute (HVI)
- Focus Area NanoScale of the Freie Universitat Berlin
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Aim: We analyzed the protein corona of thermoresponsive, poly(N-isopropylacrylamide)-or poly(Nisopropylmethacrylamide)- based nanogels. Materials & methods: Traces of protein corona detected after incubation in human serum were characterized by proteomics and dynamic light scattering in undiluted serum. Results: Apolipoprotein B-100 and albumin were the main components of the protein coronae. For dendritic polyglycerol-poly(N-isopropylacrylamide) nanogels at 37 degrees C, an increase in adsorbed immunoglobulin light chains was detected, followed by partially reversible nanogel aggregation. All nanogels in their hydrophilic state are colloidally stable in serum and bear a dysopsonin-rich protein corona. Conclusion: We observed strong changes in NG stability upon slight alterations in the composition of the protein coronae according to nanogel solvation state. Nanogels in their hydrophilic state possess safe protein coronae.
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