Journal
MOLECULES
Volume 24, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/molecules24010112
Keywords
Sophora flavescens; flavonoids; ultrasound-assisted extraction; antioxidant activity; response surface methodology
Funding
- Natural Science Foundation of Zhejiang Provincial [LR16H270001, LY17H270013]
- National Natural Science Foundation of China [81473587]
- Postdoctoral Science Foundation of China [2018M630692]
- Basic PublicWelfare Research Project of Zhejiang Province [LGN18A010001]
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The ultrasonic-assisted extraction process and antioxidant activity of flavonoids from Sophora flavescens were investigated in this study. In order to optimize the extraction of flavonoids from Sophora flavescens, the influence of extraction time, methanol concentration, ultrasonic temperature, and solvent-to-material ratio was analyzed. Results showed that the extraction yields reached a maximum with the extraction time of 30 min, methanol concentration of 80%, temperature of 80 degrees C, and solvent-to-material ratio of 26 mL/g. The flavonoids were determined by HPLC, and the mean yields of trifolirhizin, formononetin, isoxanthohumol, maackiain, and kurarinone under the optimal conditions were 2.570, 0.213, 0.534, 0.797, and 3.091 mg/g, respectively. The evaluation of vitro antioxidant activity exhibited Sophora flavescens flavonoids had a strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical-scavenging ability with IC50 of 0.984 and 1.084 mg/g, respectively. These results indicate that ultrasonic-assisted extraction is an efficient approach for the selective extraction of flavonoids, and response surface methodology further optimized the extraction.
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