Journal
MOLECULES
Volume 23, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/molecules23112866
Keywords
essential oil; extraction techniques; hops extracts; hydrodistillation; Marynka strain; microwave-assisted hydrodistillation
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Two different extraction methods were used for a comparative study of essential oils obtained from the Humulus lupulus scCO(2) (sc-supercritical) extract: microwave-assisted hydrodistillation (MAHD) and conventional hydrodistillation (HD). As a result, the best conditions for the maximum essential oil production were determined for the MAHD method at 335 W microwave power for 30 min at water to raw material ratio of 8:3. The obtained essential oils were enriched in beta-myrcene in the amount of 74.13%-89.32% (wt%). Moreover, the first application for determination of the above-mentioned volatile compounds by supercritical fluid chromatography (SFC) with photo-diode array detection (PDA) is presented, which in comparison with gas chromatography coupled with mass spectrometry (GC-MS/MS) resulted in similar values for -myrcene and -humulene in obtained samples within less than 1 min.
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