Journal
ACS CHEMICAL NEUROSCIENCE
Volume 7, Issue 5, Pages 647-661Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acschemneuro.6b00018
Keywords
M-1 muscarinic acetylcholine receptor; positive allosteric modulator
Funding
- Australian Research Council [DP110100687]
- National Health and Medicinal Research Council (NHMRC) of Australia [APP1055134, APP1049564]
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Benzoquinazolinone 1 is a positive allosteric modulator (PAM) of the M-1 muscarinic acetylcholine receptor (mAChR), which is significantly more potent than the prototypical PAM, 1-(4-methoxybenzyl)-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (BQCA). In this study, we explored the structural determinants that underlie the activity of 1 as a PAM of the M-1, mAChR. We paid particular attention to the importance of the tricyclic scaffold of compound 1, for the activity of the molecule. Complete deletion of the peripheral fused benzene ring caused a significant decrease in affinity and binding cooperativity with acetylcholine (ACh). This loss of affinity was rescued with the addition of either one or two methyl groups in the 7- and/or 8 position of the quinazolin-4(3H)-one core. These results demonstrate that the tricyclic benzo[h]quinazolin-4(3H)-one core could be replaced with a quinazolin-4(3H)-one core and maintain functional affinity. As such, the quinazolin-4(3H)-one core represents a novel scaffold to further explore M-1 mAChR PAMs with improved physicochemical properties.
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