Journal
MOLECULAR MEDICINE REPORTS
Volume 19, Issue 3, Pages 2254-2262Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2019.9862
Keywords
hepatoma; Scutellaria barbata polysaccharides; serum proteomics; two-dimensional gel electrophoresis; matrix-assisted laser desorption ionization time-of-flight mass spectrometry
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Funding
- National Natural Science Foundation Emergency Management Project [81441113]
- Research Project of the Health and Family Planning Commission of Heilongjiang Province [2017-582]
- [2015/01-2015/12]
- [2017/1-2018/12]
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The present study aimed to evaluate the antitumor effect of Scutellaria barbata polysaccharides (SBPS) in a hepatoma mouse model and examine the serum proteins involved in the tumorigenesis and SBPS treatment. A hepatoma model was established by the subcutaneous inoculation of murine hepatocellular carcinoma into Kunming mice. The treatment (once a day) lasted until the tumor weight in the model group was 1 g (7-10 days after inoculation). The sera proteins from each group were then collected and subjected to two-dimensional gel electrophoresis. Differentially expressed proteins were screened out and representatives were identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. SBPS treatment at different doses significantly inhibited hepatoma growth (all P<0.01 vs. model group). The comparative serum proteomics showed that pseudouridine synthase 1 and chain A of the signal recognition particle Alu RNA-binding heterodimer (Srp9/14) were increased in the serum of the H22 hepatoma-bearing mice, and both were reduced by SBPS treatment. Mitochondrial ribosomal protein L24 was absent from the serum of H22 hepatoma-bearing mice, and was restored by SBPS treatment to approximately the normal level. Taken together, SBPS inhibited the growth of hepatic carcinoma in mice and affected serum proteomic profiling.
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