Optimized protocols for T cell expansion and lentiviral transduction

T cells are a subset of unconventional T cells that serve a critical role in infectious diseases and various types of cancer. Cell therapy with genetically-modified T cells is regarded as a promising tool for tumor treatment. However, since T cells constitute a minority of T cells, their large-scale expansion is difficult to realize in an efficient and cost-effective manner. In the present study, based on previous studies, culture protocols for T cells were tested using different combinations of isopentenyl pyrophosphate and interleukin 2 in order to satisfy different experimental purposes. One protocol was demonstrated to be the most suitable for lentiviral transduction. These results greatly reinforce the promising prospects of using T cells in basic research and for clinical applications.