Journal
MOLECULAR BREEDING
Volume 39, Issue 1, Pages -Publisher
SPRINGER
DOI: 10.1007/s11032-018-0910-2
Keywords
Yunnan hulled wheat; HMW-GS; 1Dy12**; Marker-assistance selection
Categories
Funding
- 973 Program [2014CB138100]
- National Natural Science Foundation of China [31520103911, 31471488, 31171553]
- Doctor Foundation of Guizhou Normal University
- Guizhou Science and Technology Foundation [QKHJZLKS [2012] 21]
Ask authors/readers for more resources
High molecular weight glutenin subunits (HMW-GSs) play an important role in affecting dough viscoelasticity and extensibility. In this work, the novel HMW-GS gene 1Dy12** in Yunnan hulled wheat was cloned and characterized, and the molecular marker for identifying this gene was developed. SDS-PAGE analysis indicated that the mobility of 1Dy12** was the same as that of 1Dy12. The coding sequence of 1Dy12** was 1953bp, which was 33bp less than that of 1Dy12. 1Dy12** possessed 649 amino acid residues and showed a similar molecular structure to the published y-type subunit. It possessed four domains: a signal peptide, a conservative N-terminal domain, a large repetitive domain, and a conservative C-terminal domain. Eight cysteine residues were present in 1Dy12**, which was one more than the conserved number of cysteine residues in the y-type subunit. In vitro SDS-sedimentation tests demonstrated that 1Dy12** could bring higher SDS-sedimentation volumes than those of 1Dy10 or 1Dy12. A set of functional markers for the 1Dy12** gene was developed and validated on 36 bread wheat varieties with different Glu-1 alleles and 48 recombinant inbred lines derived by Yunnan hulled wheat and Yanzhan 1. The markers could effectively distinguish 1Dy12** from other HMW-GS genes and, thus, provide a useful tool for marker-assisted selection in wheat quality improvement programs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available