Journal
MICROCHIMICA ACTA
Volume 186, Issue 2, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-018-3214-7
Keywords
Tetramethylbenzidine; Human serum sample; Lactate oxidase; Gold nanoparticles
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Funding
- National Natural Science Foundation of China [21575165]
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The intrinsic peroxidase-like activity of hollow Prussian Blue nanoparticle-loaded with gold nanoparticles (Au@HMPB NPs) were applied to oxidize the substrate 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2 to give a blue-green coloration. The morphology of the Au@HMPB NPs and its peroxidase-mimicking activity was characterized in detail. The catalytic activity follows Michaelis-Menten kinetics and is higher than that of HMPB NPs not loaded with gold nanoparticles. The NPs were employed to detect L-lactic acid colorimetrically (at 450nm) via detection of H2O2 that is generated during enzymatic oxidation by L-lactate oxidase (LOx). The limit of detection is 4.2M. The assay was successfully applied to the quantitation of L-lactic acid in spiker human serum samples.Gold nanoparticle-loaded hollow Prussian Blue nanoparticles (Au@HMPB NPs) with peroxidase-like catalytic activity can oxidize the substrate 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2. The nanoparticles were applied for the detection of L-lactic acid through detection of H2O2 that is generated by L-lactate oxidase (LOx) catalyzed oxidation of L-lactic acid.
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