Journal
MICROCHIMICA ACTA
Volume 185, Issue 12, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-018-3109-7
Keywords
MOF; Human saliva; Mass spectrometry; Metal oxide affinity chromatography; Phosphopeptide; On-column enrichment; MALDI-TOF MS; Phosphoproteome; Polydopamine; Size exclusion effect
Categories
Funding
- National Key R&D Program of China [2018YFA0507501]
- National Natural Science Foundation of China [21425518]
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A zirconium(IV)-based metal organic framework (Zr-MOF) was deposited on polydopamine-coated silica microspheres to form microspheres of type SiO2@PDA@Zr-MOF. These were packed into capillary columns for enrichment of phosphopeptides. The column was off-line coupled to both matrix-assisted laser desorption/ionization time of flight mass spectrometry and LC-ESI-MS/MS. The method has a detection limit as low as 4 fmol of -casein digest and a selectivity as high as 1:1000 (molar ratio of -casein and BSA digest). Itwas applied to the analysis of human saliva. In total, 240 endogenous phosphopeptides were identified in only 25L human saliva.A zirconium-based metal organic ramework (Zr-MOF) was modified outside of polydopamine-coated silica microspheres to form microspheres named SiO2@PDA@Zr-MOF. Then they were packed in capillary columns for selective enrichment of phosphopeptides via interaction between Zr-O clusters and phosphate groups. The pre-concentration resulted in a better detection of phosphopeptides by mass spectrometry. Tris: Tris(hydroxymethyl)aminomethane; DMF: Dimethyl Formamide; Zr-MOF: Zirconium(IV)-organic framework; MOAC: Metal oxide affinity chromatography.
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