Journal
MICROCHIMICA ACTA
Volume 186, Issue 2, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-018-3114-x
Keywords
Estrogen; Aptamer; SERS; Hybridization chain reaction; Signal amplification; Gold nanoparticle; Environment monitoring; Food safety
Categories
Funding
- NSFC [21475030]
- Open Foundation of Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization [EWPL201701]
- China Agriculture Research System-48 (CARS-48)
- Anhui Provincial Modern Argo-industry Tech. Research System [NYCYTX-2016-84]
- Fundamental Research Fund for central university [2017HGPA0162, JZ2018HGTA0205, PA2017GDQT0018]
- [2017YFF0208600]
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The authors describe an aptamer-based assay for 17-estradiol. It relies on the combined use of surface enhanced Raman scattering (SERS) and hybridization chain reaction (HCR). The aptamer against 17-estradiol is applied as the recognition probes, and this results in excellent specificity. Specific recognition of target 17-estradiol induce the freedom of DNA 2, which will open the stem-loop structure of probe 1 on the Au@Ag and form the partial dsDNA structure. With the nicking enzyme, the partial dsDNA will be hydrolyzed and the reside ssDNA on Au@Ag will form a small stem-loop structure. With the help of the other probe 2 modified Au@Ag and pre-immobilized probe 3 on the well of the microplate, an enzyme-free HCR can occur and tremendous Au@Ag can be assembled along the formed dsDNA in HCR, which can act as the excellent substrate for Raman measurement and greatly amplify the Raman signal of R6G on the Au@Ag. Afterwards, the key factor, ratio between probe 2-Au@Ag (P2) and probe1-Au@Ag (P1), affects the detection sensitivity is systematically optimized for the best sensing performance. The SERS signal of R6G, best measured at 1651cm(-1),increases linearly in the wide range from 1 pM to 10nM. The detection limit can be as low as 0.1 pM.
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