Journal
MICROBIAL CELL FACTORIES
Volume 17, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12934-018-1047-z
Keywords
Periplasmic secretion; Antibody fragments; Riboswitches; Codon usage; Signal peptides
Categories
Funding
- IB Catalyst Award
- EPSRC CDT grant [EP/I033270/1]
- BBSRC DTP grant [BB/M011208/1]
- BBSRC [BB/K014773/1]
- UKRI research council BBSRC [BB/M011259/1]
- UKRI research council Innovate UK [131836]
- BBSRC [1618786, BB/K014773/1, BB/L004402/1, BB/M011259/1] Funding Source: UKRI
- EPSRC [EP/I033270/1] Funding Source: UKRI
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BackgroundThe secretion of recombinant disulfide-bond containing proteins into the periplasm of Gram-negative bacterial hosts, such as E. coli, has many advantages that can facilitate product isolation, quality and activity. However, the secretion machinery of E. coli has a limited capacity and can become overloaded, leading to cytoplasmic retention of product; which can negatively impact cell viability and biomass accumulation. Fine control over recombinant gene expression offers the potential to avoid this overload by matching expression levels to the host secretion capacity.ResultsHere we report the application of the RiboTite gene expression control system to achieve this by finely controlling cellular expression levels. The level of control afforded by this system allows cell viability to be maintained, permitting production of high-quality, active product with enhanced volumetric titres.ConclusionsThe methods and systems reported expand the tools available for the production of disulfide-bond containing proteins, including antibody fragments, in bacterial hosts.
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