Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 8, Issue 35, Pages 22849-22859Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b06403
Keywords
silk fibroin; laminin peptide; surface functionalization; biomaterials; tissue engineering; transdifferentiation; neuron-like stem cells
Funding
- JNCASR, the Department of Biotechnology (DBT), Government of India, New Delhi [BT/03/IYBA/2010]
- Science and Engineering Research Board (SERB), the Department of Science and Technology (DST), Government India [SB/S1/OC-47/2013]
- Council of Scientific & Industrial Research (CSIR), India
- Translational Center on Biomaterials for Orthopedic and Dental Applications Department of Biotechnology (DBT)
- Stem cell task force of DBT, Government of India
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Surface interactions at the biomaterial cellular interface determine the proliferation and differentiation of stem cells. Manipulating such interactions through the surface chemistry of scaffolds renders control over directed stem cell differentiation into the cell lineage of interest. This approach is of central importance for stem cell-based tissue engineering and regenerative therapy applications. In the present study, silk fibroin films (SFFs) decorated with integrin-binding laminin peptide motifs (YIGSR and GYIGSR) were prepared and employed for in vitro adult stem cell-based neural tissue engineering applications. Functionalization of SFFs with short peptides showcased the peptide sequence and nature of functionalization-dependent differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs). Intriguingly, covalently functionalized SFFs with GYIGSR hexapeptide (CL2-SFF) supported hMSC proliferation and maintenance in an undifferentiated pluripotent state and directed the differentiation of hMSCs into neuron-like cells in the presence of a biochemical cue, on-demand. The observed morphological changes were further corroborated by the up-regulation of neuronal-specific marker gene expression (MAP2, TUBB3, NEFL), confirmed through semiquantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The enhanced proliferation and on-demand directed differentiation of adult stem cells (hMSCs) by the use of an economically viable short recognition peptide (GYIGSR), as opposed to the integrin recognition protein laminin, establishes the potential of SFFs for neural tissue engineering and regenerative therapy applications.
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