In vitro Cytotoxicity, MMP and ROS activity of green synthesized nickel oxide nanoparticles using extract of Terminalia chebula against MCF-7 cells

Nanotechnology has been offered the prospect for the development of novel nanomaterials with countless potential applications in natural sciences and clinical pharmaceutical. This study described the analysis of green synthesized nickel oxide nanoparticles (NPs) using the extract of Terminalia chebula. The Green synthesized NiO NPs were characterized by Transmission Electron Microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-rays Analysis (EDAX) and ultraviolet-visible spectroscopy (UV-Vis). Green synthesized NiO NPs displayed the toxicity to breast cancerous cells in a dose-dependent way from 0 to 100 mu g/mL showing noticeable cell viability, Reactive oxygen species (ROS) activity and liberating of mitochondrial membrane potential (MMP). The statistical scrutiny was also done on the experimental outcomes to check the value and precision of the effects, with p-values <0.05 selected as significant. The planned approach deliberates the NiO NPs as a function of phenolic extracts of T. chebula with vast potential for several biological and biomedical applications. (C) 2018 Elsevier B.V. All rights reserved.