Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 8, Issue 7, Pages 4378-4384Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b10792
Keywords
tetrahedral DNA nanostructures; tumor targeting; dual-modality imaging; single-photon emission computed tomography; near-infrared fluorescence
Funding
- Ministry of Science and Technology of China [2012CB825805, 2012CB932603, 2013CB933802]
- National Science Foundation of China [21390414, 21227804, 91313302, 61378062, U1232114, 21329501, 31371015, 11405013, 11575278, 21505148]
- Shanghai Municipal Commission for Science and Technology [13NM1402300]
- Strategic Leading Science & Technology Program [XDA 02030000]
- Youth Innovation Promotion Association, CAS
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In this work, we have developed multiple-armed DNA tetrahedral nanostructures (TDNs) for dual-modality in vivo imaging using near-infrared (NIR) fluorescence and single-photon emission computed tomography (SPECT). We found that the presence of arm strands in TDNs remarkably enhanced their in vitro stability, allowing them to stay intact for at least 12 h in serum. By using NIR fluorescence imaging, we evaluated in mice the pharmacokinetics of TDNs, which exhibited distinctly different in vivo biodistribution patterns compared with those of double-stranded (ds)DNA. We also noticed that TDNs had twofold longer circulation time in the blood system than that of dsDNA. With the use of multiple armed TDNs, we could precisely anchor an exact number of functional groups including tumor-targeting folic acid (FA), NIR emitter Dylight 755, and radioactive isotope Tc-99m on prescribed positions of TDNs, which showed the capability of targeted imaging ability in cancer cells. Furthermore, we realized noninvasive tumor-targeting imaging in tumor-bearing mice by using both NIR and SPECT modalities.
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