Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 8, Issue 40, Pages 26648-26656Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b11371
Keywords
hydrogels; thiol-ene click chemistry; poly(ethylene glycol); hyperbranched polymer; hyaluronic acid; antibacterial; wound care
Funding
- Irish Research Council [EBPPG/2014/70, EBPPG/2015/131]
- Science Foundation Ireland (SFI) [13/IA/1962, 10/IN.1/B2981(T)]
- Health Research Board (HRB) of Ireland [HRA-POR-2013-412]
- DEBRA Ireland [H2020-MSCA-ITN-2014]
- University College Dublin (UCD)
- National University of Ireland Galway (NUI Galway)/CURAM
- Irish Research Council (IRC) [EBPPG/2015/131] Funding Source: Irish Research Council (IRC)
- Engineering and Physical Sciences Research Council [EP/E042619/1, EP/E042619/2] Funding Source: researchfish
- Science Foundation Ireland (SFI) [10/IN.1/B2981(T)] Funding Source: Science Foundation Ireland (SFI)
- EPSRC [EP/E042619/2, EP/E042619/1] Funding Source: UKRI
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A multifunctional branched copolymer was synthesized by Reversible Addition Fragmentation Chain Transfer polymerization (RAFT) of poly(ethylene glycol) diacrylate (PEGDA M-n = 575) and poly(ethylene glycol) methyl methacrylate (PEGMEMA M-n = SOO) at a feed molar ratio of 50:50. Proton nuclear magnetic resonance spectroscopy (H-1 NMR) confirmed a hyperbranched molecular structure and a high degree of vinyl functionality. An in situ cross-linkable hydrogel system was generated via a click thiol-ene-type Michael addition reaction of vinyl functional groups from this PEGDA/PEGMEMA copolymer system in combination with thiol-modified hyaluronic acid. Furthermore, encapsulation of antimicrobial silver sulfadiazine (SSD) into the copolymer system was conducted to create an advanced antimicrobial wound care dressing. This hydrogel demonstrated a sustained antibacterial activity against the bacterial strains Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli in comparison to the direct topical application of SSD. In addition, in vitro toxicology evaluations demonstrated that this hydrogel with low concentrations of encapsulated SSD supported the survival of embedded human adipose derived stem cells (hADSCs) and inhibited growth of the aforementioned pathogens. Here we demonstrate that this hydrogel encapsulated with a low concentration (1.0% w/v) of SSD can be utilized as a carrier system for stem cells with the ability to inhibit growth of pathogens and without adverse effects on hADSCs.
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