Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 8, Issue 7, Pages 4489-4499Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b00158
Keywords
cerium oxide nanoparticles; mesenchymal stem cells; angiogenesis; vasculogenesis; tissue engineering bone
Funding
- National Natural Science Foundation of China [81471782]
- National High-tech R&D Program of China (863 Program) [2015AA020315]
- National Key Technology Research and Development Program of China [2012BAI42G01]
Ask authors/readers for more resources
Insufficient blood perfusion is one of the critical problems that hamper the clinical application of tissue engineering bone (TEB). Current methods for improving blood vessel distribution in TEB mainly rely on delivering exogenous angiogenic factors to promote the proliferation, migration, differentiation, and vessel formation of endothelial cells (ECs) and/or endothelial progenitor cells (EPCs). However, obstacles including limited activity preservation, difficulty in controlled release, and high cost obstructed the practical application of this strategy. In this study, TEB scaffold were modified with cerium oxide nanoparticles (CNPs) and the effects of CNPs existed at the scaffold surface on the growth and paracrine behavior of mesenchymal stem cells (MSCs) were investigated. The CNPs could improve the proliferation and inhibit the apoptosis of MSCs. Meanwhile, the interaction between the cell membrane and the nanoparticle surface could activate the calcium channel of MSCs leading to the rise of intracellular free Ca2+ level, which subsequently augments the stability of HIF-1 alpha. These chain reactions finally resulted in high expression of angiogenic factor VEGF. The improved paracrine of VEGF could thereby promote the proliferation, differentiation, and tube formation ability of EPCs. Most importantly, in vivo ectopic bone formation experiment demonstrated this method could significantly improve the blood vessel distribution inside of TEB.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available