Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 8, Issue 6, Pages 4137-4148Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b11879
Keywords
electrophoretic deposition; mesoporous silica nanoparticles (MSNs); dexamethasone; poly(L-lactic acid)/poly(epsilon-caprolactone) scaffold; bone tissue engineering
Funding
- National Natural Science Foundation of China [31271028, 31570984]
- Shanghai Municipal Education Commission [13ZZ051]
- Science and Technology Commission of Shanghai Municipality [15540723400]
- Open Foundation of State Key Laboratory for Modification of Chemical Fibers and Polymer Materials [LK1416]
- Chinese Universities Scientific Fund [CUSF-DH-D-2013008]
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The incorporation of microcarriers as drug delivery vehicles into polymeric scaffold for bone regeneration has aroused increasing interest. In this study, the aminated mesoporous silica nanoparticles (MSNs-NH2) were prepared and used as microcarriers for dexamethasone (DEX) loading. Poly(L-lactic acid)/poly(epsilon-caprolactone) (PLLA/PCL) nanofibrous scaffold was fabricated via thermally induced phase separation (TIPS) and served as template, onto which the drug-loaded MSNs-NH2 nanoparticles were deposited by electrophoretic deposition (EPD). The physicochemical and release properties of the prepared scaffolds (DEX@MSNs-NH2/PLLA/PCL) were examined, and their osteogenic activities were also evaluated through in vitro and in-vivo studies. The release of DEX from the scaffolds revealed an initial rapid release followed by a slower and sustained one. The in vitro results indicated that the DEX@MSNs-NH2/PLLA/PCL scaffold exhibited good bio-compatibility to rat bone marrow-derived mesenchymal stem cells (BMSCs). Also, BMSCs cultured on the DEX@MSNs-NH2/PLLA/PCL scaffold exhibited a higher degree of osteogenic differentiation than those cultured on PLLA/PCL and MSNs-NH2/PLLA/PCL alkaline phosphatase (ALP) activity, mineralized matrix formation, and osteocalcin (OCN) expression. Furthermore, the in vivo results in a calvarial defect model of Sprague-Dawley (SD) rats demonstrated that the DEX@MSNs-NH2/PLLA/PCL scaffold could significantly promote calvarial defect healing compared with the PLLA/PCL scaffold. Thus, the EPD technique provides a convenient way to incorporate osteogenic agents-containing microcarriers to polymer scaffold, and thus, prepared composite scaffold could be a potential candidate for bone tissue engineering application due to its capacity for delivery of osteogenic agents.
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