Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 262, Issue -, Pages 61-64Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2018.07.008
Keywords
Mammalian reovirus; sigma 1 protein; Indirect ELISA
Funding
- National Natural Science Foundation of China [31402204]
- Central Public-interest Scientific Institution Basal Fund [1610302016002]
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Mammalian reovirus (MRV) infects many species. Over the past decades, MRV infections in pigs have been reported, and several highly pathogenic MRV strains have recently been isolated in the United States. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) against the sigma 1 protein from a serotype 3 reovirus strain (MPC/04) was established to detect antibodies in pigs. The assay did not react with antisera against other pig pathogens and was consistent with the indirect immunofluorescence assay (IFA) and virus neutralization test (VNT). In conclusion, the assay is specific and highly sensitive, providing a method for large-scale monitoring of the serotype 3 MRV infection epidemiology in pigs.
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