Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 9, Issue 1, Pages 236-243Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b13998
Keywords
metal organic frameworks; amino acids; fluorescence biosensors; mechanism; molecular logic gate
Funding
- National Natural Science Foundation of China [21275081, 21322507]
- National Basic Research Program of China [2011CB707703]
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Most of the reported metal organic frameworks (MOFs)-based DNA sensors were developed by utilizing the different adsorption capacities of MOFs to different structural DNAs (for example, single-stranded DNAs (ssDNAs) and double-stranded DNAs (dsDNAs)) or ssDNAs with different lengths. Herein, we introduced another strategy for the design of MOFs-based biosensing platforms. We found that specific small-sized amino acids (for example, glycine and serine) could lead to the destruction of the MOFs formed by [Cu(mal)-(bpy)] center dot 2H(2)O] thus recovering the fluorescence of a fluorophore-labeled ssDNA that had been quenched by MOFs. The corresponding working mechanism was discussed. On the basis of this finding, a mix-and-detect fluorescence method was designed for the turn-on detection of specific small-sized amino acids. The feasibility of its use in real serum samples was also demonstrated. Besides biosensing applications, the discovery of amino acids-triggered destruction of MOFs can also enrich the building blocks of molecular logic gate. As an example, a biomolecular logic gate that performs OR logic operation was constructed using glycine and a DNA strand as inputs.
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