Journal
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
Volume 81, Issue 21, Pages 1142-1149Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15287394.2018.1532716
Keywords
Cyanobacterial blooms; PCR; Microcystin; Microcystis
Funding
- National Natural Science Foundation [81773393, 81502787]
- Central South University Innovation Driven Project [20170027010004]
- National Public Welfare Industrial Foundation [201503108]
- Key Research and Development Projects in Hunan Province [2018WK2013]
- Program for College students Free Exploration [502331007, ZY20170939]
- [2014EME001]
Ask authors/readers for more resources
Global proliferation of cyanobacterial blooms associated with climate change and eutrophication constitutes a serious environmental threat. In Hunan Province a freshwater pond located in Changsha City was found to contain high concentrations of cyanobacteria, however, the characteristics of these cyanobacteria at present are not known. This study thus aimed to isolate, identify the most common bloom-forming cyanobacteria in this region and determine the toxigenic characteristics of the predominant cyanobacteria. The cyanobacteria were isolated by serial dilution and identified using polymerase chain reaction (PCR). The cyanotoxins generated by the cyanobacterium were detected using high-performance liquid chromatography with an ultra-high resolution LTQ Orbitrap Velos Pro ETD mass spectrometry equipped with electrospray ionization interface (HPLC-ESI-MS). One species of cyanobacterium was isolated and identified as Microcystis sp. YFM1 according to the sequence of the 16S ribosome deoxyribonucleic acid (16S rDNA). It was found that this cyanobacterium contained microcystin synthetase B gene (mcyB) and produced three types of cyanotoxins including microcystin-LR, RR and YR. Our findings indicate that the Microcystis sp. YFM1 isolated from the freshwater pond in Hunan Province exhibits unique characteristics distinguishable from other known cyanobacteria.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available