Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 8, Issue 47, Pages 32202-32210Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b11319
Keywords
gold nanorod; hyaluronate; antibody; transdermal delivery; skin cancer
Funding
- National Research Foundation (NRF) - Korean government (MEST) [2012M3A9C6049791, 2015R1A2A1A15053779]
- Korea Health Technology R&D Project of the Ministry of Health and Welfare [HI15C1817]
- National Research Foundation of Korea [2015R1A2A1A15053779] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Noninvasive transdermal delivery is a promising method with distinct advantages including patient compliance over other delivery routes. Here, hyaluronate gold nanorod/death receptor 5 antibody (HA-AuNR/DR5 Ab) complex was developed for transdermal theranosis of skin cancer. The successful formation of the complex was corroborated by H-1 nuclear magnetic resonance, UV vis spectroscopy, dynamic light scattering, zeta potential, and transmission electron microscopy. In vitro biological activity of the complex was verified by ELISA and MTT assay using HCT116 cancer cells. In addition, in vivo photoacoustic imaging and two-photon microscopy clearly-visualized the transdermal delivery of HA-AuNR/DR5 Ab complex through the inevitable barrier of stratum corneum in the skin. Furthermore, in vivo antitumor effect on skin cancer model mice was confirmed from statistically significant decrease of tumor-reflecting luciferase expression levels and apoptotic signals in terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Taken together, we could confirm the feasibility of HA-AuNR/DR5 Ab complex as a novel theranostic platform for noninvasive transdermal treatment of skin cancers.
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