Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Volume 1847, Issue 10, Pages 1101-1112Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2015.05.018
Keywords
Mg2+ coordination; ATPase inhibition; F-type ATPase; Bacillus subtilis
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Funding
- Japan Society for the Promotion of Science (JSPS-fellowship) [P13705]
- Grants-in-Aid for Scientific Research [26104517, 25118509, 25251019] Funding Source: KAKEN
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F-type ATP synthases, central energy conversion machines of the cell synthesize adenosine triphosphate (ATP) using an electrochemical gradient across the membrane and, reversely, can also hydrolyze ATP to pump ions across the membrane, depending on cellular conditions such as ATP concentration. To prevent wasteful ATP hydrolysis, mammalian and bacterial ATP synthases possess different regulatory mechanisms. In bacteria, a low ATP concentration induces a conformational change in the a subunit from the down- to up-states, which inhibits ATP hydrolysis. Moreover, the conformational change of the epsilon subunit depends on Mg2+ concentration in some bacteria such as Bacillus subtilis, but not in others. This diversity makes the a subunit a potential target for antibiotics. Here, performing molecular dynamics simulations, we identify the Mg2+ binding site in the epsilon subunit from B. subtilis as E59 and E86. The free energy analysis shows that the first-sphere bi-dentate coordination of the Mg2+ ion by the two glutamates is the most stable state. In comparison, we also clarify the reason for the absence of Mg2+ dependency in thee subunit from thermophilic Bacillus PS3, despite the high homology to that from B. subtilis. Sequence alignment suggests that this Mg2+ binding motif is present in the epsilon subunits of some pathogenic bacteria. In addition we discuss strategies to stabilize an isolated epsilon subunit carrying the Mg2+ binding motif by site directed mutagenesis, which also can be used to crystallize Mg2+ dependent epsilon subunits in future. (C) 2015 Elsevier B.V. All rights reserved.
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