Green biosynthesis of gold nanoparticles using Chenopodium formosanum shell extract and analysis of the particles' antibacterial properties

BACKGROUNDVarious physical and chemical methods for synthesis of metal nanoparticles have had some drawbacks. Therefore, green synthesis of gold nanoparticles (Au NPs) has became one of the most crucial emerging areas of nanobiotechnology. In the present study, plant-mediated synthesis of Au NPs was performed using Djulis (Chenopodium formosanum) shell extract as a reducing and stabilizing agent. RESULTSReaction parameters were manipulated to optimize the Au NPs using a UV-visible spectrophotometer. Optimized Au NPs with a surface plasmon resonance band at 533nm were prepared using a 744 mu gmL(-1) extract and a solution of pH2.62 chloroauric acid (HAuCl(4)3H(2)O) at 40 degrees C. High-resolution transmission electron microscopy (HR-TEM) results indicated that most of the resultant Au NPs were spherical in shape and exhibited a mean size of 86nm. Energy-dispersive X-ray spectroscopy (EDS), and selected area electron diffraction (SAED), and X-ray diffraction (XRD) confirmed the elemental gold and crystalline nature of the resultant NPs. FTIR spectrum analysis indicated the critical role of phenolic groups in the reduction of Au3+ ions and stabilization of the formed Au NPs. Moreover, the synthesized Au NPs possessed antibacterial activity for Escherichia coli and Staphylococcus aureus. CONCLUSIONIn this study, Au NPs were synthesized with high efficiency and stability using Djulis shell extract, and related antibacterial applications were demonstrated. (c) 2019 Society of Chemical Industry