Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 8, Issue 28, Pages 17878-17884Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b05471
Keywords
aggregation-induced emission; long-term cell tracking; bone repair; stem cell; osteogenic differentiation
Funding
- Ministry of Science and Technology of China [2013CB834702, 2012CB619100]
- National Science Foundation of China [21490571, 51273053, 51232002]
- Natural Science Foundation of Guangdong Province [2016A030313852, 2016A030312002]
- China Postdoctoral Science Foundation [2015M580716, 2016T90778]
- Fundamental Research Funds for the Central Universities [2015ZY013, 2015ZZ104]
- Guangdong Natural Science Funds for Distinguished Young Scholar Grant [2014A030306035]
- Guangdong Innovative Research Team Program [201101C0105067115]
- [ITC-CNERC14S01]
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Bone marrow-derived mesenchymal stem cells (BMSCs) have shown great potential for bone repair due to their strong proliferation ability and osteogenic capacity. To evaluate and improve the stem cell-based therapy, long-term tracking of stem cell differentiation into bone-forming osteoblasts is required. However, conventional fluorescent trackers such as fluorescent proteins, quantum dots, and fluorophores with aggregation-caused quenching (ACQ) characteristics have intrinsic limitations of possible interference with stem cell differentiation, heavy metal cytotoxicity, and self-quenching at a high labeling intensity. Herein, we developed aggregation-induced emission nanoparticles decorated with the Tat peptide (AIE-Tat NPs) for long-term tracking of the osteogenic differentiation of mouse BMSCs without interference of cell viability and differentiation ability. Compared with the ability of the commercial Qtracker 655 for tracking of only 6 passages of mouse BMSCs, ATE-Tat NPs have shown a much superior performance in long-term tracking for over 12 passages. Moreover, long-term tracking of the osteogenic differentiation process of mouse BMSCs was successfully conducted on the biocompatible hydroxyapatite scaffold, which is widely used in bone tissue engineering. Thus, AIE-Tat NPs have promising applications in tracking stem cell fate for bone repair.
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