Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Volume 1847, Issue 10, Pages 1153-1165Publisher
ELSEVIER
DOI: 10.1016/j.bbabio.2015.05.020
Keywords
Carotenoid deficiency; Cyanobacterial photosynthesis; Phycobilisome; Photosynthetic complexes; Time-resolved fluorescence
Categories
Funding
- Sandwich PhD program of Wageningen University
- HARVEST Marie Curie Research Training Network [PITN-GA-2009-238017]
- Hungarian Scientific Research Fund [OTKA K108411, K112688]
- Social Renewal Operational Programs [TAMOP-4.2.2/B-10/1-2010-0012, TAMOP-4.2.2.A-11/1/KONV-2012-0047]
- A*STAR Singapore (NIH-A*STAR) [TET_10-1-2011-0279]
- Grant Agency of the Czech republic [P501/12/G055]
- bilateral project of Academy of Science of the Czech Republic and Hungary [HU/2013/06]
- National Program of Sustainability I [LO1416]
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In photosynthetic organisms, carotenoids (carotenes and xanthophylls) are important for light harvesting, photoprotection and structural stability of a variety of pigment-protein complexes. Here, we investigated the consequences of altered carotenoid composition for the functional organization of photosynthetic complexes in wild-type and various mutant strains of the cyanobacterium Synechocystis sp. PCC 6803. Although it is generally accepted that xanthophylls do not play a role in cyanobacterial photosynthesis in low-light conditions, we have found that the absence of xanthophylls leads to reduced oligomerization of photosystems land II. This is remarkable because these complexes do not bind xanthophylls. Oligomerization is even more disturbed in crtH mutant cells, which show limited carotenoid synthesis; in these cells also the phycobilisomes are distorted despite the fact that these extramembranous light-harvesting complexes do not contain carotenolds. The number of phycocyanin rods connected to the phycobilisome core is strongly reduced leading to high amounts of unattached phycocyanin units. In the absence of carotenoids the overall organization of the thylakoid membranes is disturbed: Photosystem II is not formed, photosystem I hardly oligomerizes and the assembly of phycobilisomes remains incomplete. These data underline the importance of carotenoids in the structural and functional organization of the cyanobacterial photosynthetic machinery. (C) 2015 Elsevier B.V. All rights reserved.
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