Journal
JOURNAL OF PROTEOME RESEARCH
Volume 18, Issue 3, Pages 947-959Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.8b00647
Keywords
extracellular vesicles; exosomes; microvesicles; iTRAQ; proteomics; adhesion
Categories
Funding
- NIH [U19 CA197514, U54 CA217450, R01 GM117916, R01 CA206458, UL1 TR002243, T32 CA119925, F32 CA217064]
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Extracellular vesicles (EVs) are important mediators of cell cell communication due to their cargo content of proteins, lipids, and RNAs. We previously reported that small EVs (SEVs) called exosomes promote directed and random cell motility, invasion, and serum-independent growth. In contrast, larger EVs (LEVs) were not active in those assays, but might have unique functional properties. In order to identify protein cargos that may contribute to different functions of SEVs and LEVs, we used isobaric tags for relative and absolute quantitation (iTRAQ) liquid chromatography (LC) tandem mass spectrometry (MS) on EVs isolated from a colon cancer cell line. Bioinformatics analyses revealed that SEVs are enriched in proteins associated with cell cell junctions, cell matrix adhesion, exosome biogenesis machinery, and various signaling pathways. In contrast, LEVs are enriched in proteins associated with ribosome and RNA biogenesis, processing, and metabolism. Western blot analysis of EVs purified from two different cancer cell types confirmed the enrichment of cell matrix and cell cell adhesion proteins in SEVs. Consistent with those data, we found that cells exhibit enhanced adhesion to surfaces coated with SEVs compared to an equal protein concentration of LEVs. These data suggest that a major function of SEVs is to promote cellular adhesion.
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