Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 8, Issue 4, Pages 2833-2839Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b11567
Keywords
peptide self-assembly; nanozyme; amyloid-beta peptide; catalytically self-propagating assembly; peptide-based biosensing
Funding
- National Natural Science Foundation of China [21235003, 81501554, J1103512, J1210026]
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In this work, a new strategy of biosensor design is developed based on the assembly of amyloid beta and its multiple interactions with other bioactive species. These interactions can enable amyloid beta peptide as a multifunctional sensing element, so the immobilization of sensing probe and the step-by-step modification of the sensing interface have all been dispensed with. Instead, the kinetics of the assembly of a peptide-based catalytic network serves to convert the quantity of analyte into amplified signal readout. The designed dynamic assembling and biosensing system has also been successfully applied in detecting the activity of polyglutamylation, an essential post translation modification controlling cell skeleton and cell cycle, in biological complex samples. Further studies reveal that the serum abundance of a polyglutamylase, tubulin tyrosine ligase-like protein 12, may show parallel with the degree of development of prostate cancer and the discrimination between early cancerous development and benign conditions. And the obtained result is more distinct than that based on PSA detection, the current gold standard. This study may also point to the prospective of extending this design strategy to broader range of biosensing applications in the future.
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