Journal
JOURNAL OF PLANT PHYSIOLOGY
Volume 232, Issue -, Pages 12-22Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.jplph.2018.10.025
Keywords
cAMP; Adenylyl cyclase; Arabidopsis thaliana; Botrytis cinerea; Golovinomyces orontii; Pseudomonas syringae pv. tomato
Categories
Funding
- King Abdullah University of Science and Technology
- Royal Society
- Biotechnology and Biological Science Research Council [BB/L019345/1, BB/M017982/1]
- Fondo di Ateneo per la Ricerca di Base 2015 - University of Perugia
- National Natural Science Foundation of China [31850410470]
- Zhejiang Provincial Natural Science Foundation of China [Q19C130001]
- BBSRC [BB/L019345/1, BB/M017982/1] Funding Source: UKRI
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Adenylyl cyclases (ACs) catalyze the formation of the second messenger cAMP from ATP. Here we report the characterization of an Arabidopsis thaliana leucine-rich repeat (LRR) protein (At3g14460; AtLRRAC1) as an adenylyl cyclase. Using an AC-specific search motif supported by computational assessments of protein models we identify an AC catalytic center within the N-terminus and demonstrate that AtLRRAC1 can generate cAMP in vitro. Knock-out mutants of AtLRRAC1 have compromised immune responses to the biotrophic fungus Golovinomyces orontii and the hemibiotrophic bacteria Pseudomonas syringae, but not against the necrotrophic fungus Botrytis cinerea. These findings are consistent with a role of cAMP-dependent pathways in the defense against biotrophic and hemibiotrophic plant pathogens.
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