Clonal relationship and the association of the ST218 strain harboring blaOXA-72 gene to mortality in carbapenem-resistant Acinetobacter baumannii bacteremia

Background/purpose: In 2017, the World Health Organization categorized carbapenem-resistant Acinetobacter baumannii (CRAB) as a priority 1, critical antibiotic-resistant bacteria. This study analyzed the clinical outcomes and investigated the molecular epidemiology of CRAB bacteremia in a medical center in Northern Taiwan. Methods: We collected 62 blood isolates from patients with CRAB bacteremia from January 2014 to December 2015 at MacKay Memorial Hospital and determined the clonal relationship using the PCR-based technique for molecular epidemiology. Medical charts were reviewed for clinical outcomes. Results: Fifty-six isolates harbored the bla(OXA-51-like) and bla(OXA-23-like) carbapenemase genes, 4 isolates harbor the bla(OXA-51-like) and bla(OXA-24-like) carbapenemase genes and 2 isolates harbored only the bla(OXA-51-like) gene. After sequencing, all four isolates of bla(OXA-24-like) carbapenemase gene were confirmed to be isolates of bla(OXA-72) carbapenemase genes. In multivariate analysis in the 60 patients, the independent mortality risk factors of CRAB bacteremia included >= 65 years (elderly) (Odds ratio, 4.04, 95% CI, 1.10-14.83, p = 0.035), chronic kidney disease (4.36, 1.14-16.72, p = 0.032). Isolates harboring the bla(OXA-72) gene had the same sequence type (ST218) and PFGE pulsotype raising the possibility of intra-hospital transmission, and all infected patients died. Conclusion: This study showed the clonal relationship of isolates harboring the carbapenemase gene in CRAB bacteremia. Patients with the ST218 strain harboring bla(OXA-72) gene had high mortality. This warrants further research to determine the mechanism of virulence and risk factors in order to reduce mortality. Copyright (C) 2018, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC.