Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 155, Issue -, Pages 37-41Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2018.11.009
Keywords
XTT; Formazan; Fusarium fujikuroi; Gibberellic acid; Biomass; Fermentation
Categories
Funding
- Zhejiang Provincial Natural Science Foundation of China [LQ19C010002]
- Educational Commission of Zhejiang Province, China [Y201738062]
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A colorimetric assay has been developed for quantitative analysis of active biomass of Fusarium fujikuroi, based on the reduction of the tetrazolium salt 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) when menadione was present as an electron coupling agent. The optimum assay-conditions were set as 200 mu g/ml XTT, 5 mu M menadione and one-hour reaction time. Under these settings, the produced formazan displayed a linear relationship with F. fujikuroi biomass. This method was subsequently applied to evaluate the cell growth behavior, which showed a positive correlation with the carbon source consumption and gibberellin biosynthesis under the industrial fermentation conditions. Our results showed that the XTT-menadione assay is a valuable tool in analyzing the industrial fermentation process of F. fujikuroi, especially when the medium contains insoluble and complex components.
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