Journal
JOURNAL OF FOOD AND DRUG ANALYSIS
Volume 27, Issue 2, Pages 494-501Publisher
FOOD & DRUG ADMINSTRATION
DOI: 10.1016/j.jfda.2018.12.010
Keywords
Osteoporosis; Vitamin D metabolites; Clinical specimen; Derivatization; Vegetarian
Categories
Funding
- Buddhist Tzu-Chi General Hospital [TCMMP105-13-02]
- Tzu Chi University
- Ministry of Science and Technology, Taiwan [MOST 105-2113-M-320-002]
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Vitamin D is responsible for multiple metabolic functions in humans. Rickets are the most common disease caused by vitamin D deficiency. It is caused by poor calcium intake resulting in poor serum-ionized calcium. The purpose of this study is to develop a rapid, sensitive, and feasible method to determine the 25-hydroxy-vitamin D3 (25(OH)D3) levels in blood samples for clinical assessment. In this study, gas chromatography coupled mass spectrometry with trimethylsilyl derivatization (TMS-GC-MS) is the most suitable protocol for quantitative analyses of 25(OH)D3. Performance of method was evaluated and compared with liquid chromatography and immunoassay. Method validation has been carried out with plasma specimens. The limit of quantitation of TMS-GC-MS method is 1.5 ppb with good linear correlation. Furthermore, the dietary intake and nutritional status of vegetarian and non-vegetarians in Taiwan were assessed by our validated method. As a result, this vitamin D nutrition survey demonstrates that most Taiwanese people have insufficient vitamin D. Due to dietary habits; the male vegans may have the highest risk of vitamin D deficiency. Copyright (C) 2019, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.
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