Journal
JOURNAL OF DAIRY SCIENCE
Volume 102, Issue 2, Pages 961-975Publisher
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2018-14899
Keywords
angiotensin-I-converting enzyme (ACE)-inhibitory peptide; proteinase; Lactobacillus helveticus; Caco-2 cell; bioavailability
Funding
- National Natural Science Foundation of China [31571852, 31471598, 31671869, 31601487]
- Natural Science Foundation of Jiangsu Province [BK20151544]
- 333 Project Foundation of Jiangsu Province [BRA2017450]
Ask authors/readers for more resources
Lactobacillus helveticus LB 10 proteinases immobilized with sodium alginate were used to hydrolyze whey protein to produce angiotensin-I-converting enzyme (ACE)-inhibitory peptides. The generated hydrolysates were tested for ACE-inhibitory activity and for their ability to be transported across Caco-2 cell rnoriolayers. Using a response surface method, we determined that a proteinase concentration of 7.55 mg/mL, sodium alginate concentration of 2.03 g/100 mL, and glutaraldehyde concentration of 0.39% were found to be the optimal immobilization conditions. Compared with free proteinase, the immobilized proteinase had significantly higher pH, thermal and storage stability, and reusability. Whey protein hydrolysates were fractionated by gel filtration chromatography and ACE-inhibitory peptide mixtures were transported across Caco-2 cell monolayers in a human intestinal-absorption model. The di- and tripeptides KA, EN, DIS, EVD, LF, AIV, and VFK (half-maximal inhibitory concentrations (mean +/- standard deviation) of 1.24 +/- 0.01, 1.43 +/- 0.04, 1.59 +/- 0.27, 1.32 +/- 0.05, 1.60 +/- 0.39, 2.66 +/- 0.02, and 1.76 +/- 0.09 mmol/L, respectively) were detected on the basolateral side of the Caco-2 cell monolayer using ultra-performance liquid chromatography-tandem mass spectrometry. These results highlight that ACE-inhibitory peptides are present on the basolateral side of the Caco-2 cell model after transportation of whey protein hydrolysate across the Caco-2 cell membrane.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available