Journal
JOURNAL OF COMPARATIVE NEUROLOGY
Volume 527, Issue 5, Pages 942-956Publisher
WILEY
DOI: 10.1002/cne.24557
Keywords
amygdala; CAMKII alpha; electron microscopy; RRID AB_2313606; RRID AB_2313686; RRID AB_2336656; RRID AB_2637031; RRID AB_447192; RRID RGD_734476; thalamus; tract-tracing
Categories
Funding
- Efficacy and Mechanism Evaluation Programme [OD011132]
- National Institute of Mental Health [MH107239]
- NIH/ORIP Yerkes Center [P51 OD011132]
- NIMH [R01MH107239]
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One of the main subcortical inputs to the basolateral nucleus of the amygdala (BL) originates from a group of dorsal thalamic nuclei located at or near the midline, mainly from the central medial (CMT), and paraventricular (PVT) nuclei. Although similarities among the responsiveness of BL, CMT, and PVT neurons to emotionally arousing stimuli suggest that these thalamic inputs exert a significant influence over BL activity, little is known about the synaptic relationships that mediate these effects. Thus, the present study used Phaseolus vulgaris-leucoagglutinin (PHAL) anterograde tracing and electron microscopy to shed light on the ultrastructural properties and synaptic targets of CMT and PVT axon terminals in the rat BL. Virtually all PHAL-positive CMT and PVT axon terminals formed asymmetric synapses. Although CMT and PVT axon terminals generally contacted dendritic spines, a substantial number ended on dendritic shafts. To determine whether these dendritic shafts belonged to principal or local-circuit cells, calcium/calmodulin-dependent protein kinase II (CAMKII alpha) immunoreactivity was used as a selective marker of principal BL neurons. In most cases, dendritic shafts postsynaptic to PHAL-labeled CMT and PVT terminals were immunopositive for CaMKII alpha. Overall, these results suggest that CMT and PVT inputs mostly target principal BL neurons such that when CMT or PVT neurons fire, little feed-forward inhibition counters their excitatory influence over principal cells. These results are consistent with the possibility that CMT and PVT inputs constitute major determinants of BL activity.
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