Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1104, Issue -, Pages 134-140Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2018.11.017
Keywords
Avermectins; Milbemycines; LC-MS/MS; Fish; QuEChERS
Ask authors/readers for more resources
In this study, the simultaneous determination of avermectins (emamectin, eprinomectin, abamectin, doramectin and ivermectin) and milbemycines (moxidectin) in fish tissue with LC-ESI-MS/MS, was studied. Optimum chromatographic separation of target analytes was achieved using a Waters Acquity UPLC BEH C-18 (100 mm x 2.1 mm, 1.7 mm) analytical column, operated at 40 degrees C and the composition of the mobile phase used was (A): ACN-MeOH (0.1% HCOOH) (1:1) and (B): 1 mM HCOONH4 (0.1% HCOOH). Various mobile phases were tested and the effect of the mobile phase composition on the analytes ionization was thoroughly examined in an extensive ionization study, aiming to increase the analytes' sensitivity. Deuterated ivermectin (IVR-d(2)) was used as an internal standard (IS). Avermectins and milbemycine's extraction from the fish matrix was conducted with acidified ACN (0.1% HCOOH), followed by QuEChERS methodology. The method developed herein was validated according to the European Legislation requirements (Commission Decision 657/2002/EC) and recoveries ranged from 86 to 106% for all target analytes, with relative standard deviations < 20%. LODs ranged from 0.07 mu g/kg (emamectin) to 1.3 mu g/kg (doramectin), indicating the excellent sensitivity of the method. The developed methodology was successfully applied to fish samples obtained from aquaculture.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available